Difference between revisions of "Part:BBa K4621045"
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| − | + | Pro1008 contains the promoter（BBa_K3880007） of GQS52_24880 in SCUT-3 without 5’-UTR. The fusion promoter composed of Pro1008 and chitin-inducible promoter can control the gene expression switch while controlling its expression intensity. | |
| + | Usage, Biology and Characterization | ||
| + | Pro1008 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1008 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation. | ||
Revision as of 13:06, 5 October 2023
Pro1008 contains the promoter（BBa_K3880007） of GQS52_24880 in SCUT-3 without 5’-UTR. The fusion promoter composed of Pro1008 and chitin-inducible promoter can control the gene expression switch while controlling its expression intensity. Usage, Biology and Characterization Pro1008 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1008 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.