Difference between revisions of "Part:BBa K4759043:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Connecting GFP1-10 to the C terminus of RBM3 by linker and GFP11 to OleP, and the interacting protein will bring GFP11 to GFP1-10 close to the fluorescence, the stronger the interaction, we screened the two proteins with the strongest interaction force by bimolecular fluorescence.
+
Connecting GFP1-10 to the C terminal of RBM3 by linker and GFP11 to OleP, and the interacting protein will bring GFP11 to GFP1-10 close to the fluorescence, the stronger the interaction, we screened the two proteins with the strongest interaction force by bimolecular fluorescence.
  
  

Latest revision as of 17:58, 11 October 2023


linker-GFP1-10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 660
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Connecting GFP1-10 to the C terminal of RBM3 by linker and GFP11 to OleP, and the interacting protein will bring GFP11 to GFP1-10 close to the fluorescence, the stronger the interaction, we screened the two proteins with the strongest interaction force by bimolecular fluorescence.


Source

fusion tag

References