Difference between revisions of "Part:BBa K4907000"
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===Biology===
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<h1>Biolog</h1>
  
 
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Revision as of 10:44, 5 October 2023


sfibp-his tag


Biolog

jiantou.png | 200px

sfibp

SfIBP is a kind of antifreeze proteins (AFPs) found from Shewanella frigidimarina (1). It is capable to prevent liquid from freezing within a certain temperature range and inhibit ice growth, which is defined as Thermal Hysteresis (TH) and ice recrystallization inhibition (IRI) respectively. Additionally, the IRI activity of SfIBP is much higher beyond other AFPs.

Usage and Design

In order to measure TH and IRI activity of SfIBP, and finally verify if it is capable to solve soil frost problem, a His-tag (6×his) was added to the C-terminal of SfIBP for purification. We constructed this part and assembled it on the expression vector pET-28a(+).

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 634
    Illegal AgeI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]