Difference between revisions of "Part:BBa K4765122"
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We use this part to improve EPS production of ''E. coli'' and assessment of EPS’s adhesion ability. | We use this part to improve EPS production of ''E. coli'' and assessment of EPS’s adhesion ability. | ||
===Characterization=== | ===Characterization=== | ||
| + | ====Agarose gel electrophoresis==== | ||
| + | {| | ||
| + | | <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/pgm-gal-mscarlet.png" alt="contributed by Fudan iGEM 2023"></html> | ||
| + | |- | ||
| + | | '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | ||
| + | From left lane(1) to right lane(3) indicate the successful construction of pgmA, pgmA + galU, and pgmA + galU + mScarlet. ''' | ||
| + | |} | ||
Revision as of 06:28, 12 October 2023
ribozyme connected: pgmA + galU + mScarlet
Introduction
This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes E. coli pgmA on the downstream site and E. coli galU on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in BBa_K3331001(galU)and BBa_K3331002(pgmA). The following mScarlet is utilized to detect E. coli 's adhesion ability.
Usage and Biology
We use this part to improve EPS production of E. coli and assessment of EPS’s adhesion ability.
Characterization
Agarose gel electrophoresis
|
| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
From left lane(1) to right lane(3) indicate the successful construction of pgmA, pgmA + galU, and pgmA + galU + mScarlet. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 3285
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]