Difference between revisions of "Part:BBa K4583000"
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===Protocols=== | ===Protocols=== | ||
* <em>E. coli</em> MG1655 | * <em>E. coli</em> MG1655 | ||
| − | * 30<sup>o</sup>C, | + | * 30<sup>o</sup>C, 48h |
| + | * Equipment: | ||
| + | |||
===Characterization using fluorescent proteins=== | ===Characterization using fluorescent proteins=== | ||
===Comparison of expression time with cell density=== | ===Comparison of expression time with cell density=== | ||
Revision as of 11:53, 4 October 2023
PYU3
PYU3 is the promoter of the gene orf-0464, and it comes from Escherichia coli. It will express at the late stationary phase.
Usage and Biology
When the bacteria enter the stationary phase, the physiological state of the bacteria changes significantly. During this phase, many genes will respond to make timely adjustments. This part (BBa_K4583000) is the promoter of the gene orf-0464. Its most notable feature is that it will be expressed in the late stationary phase. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
Protocols
- E. coli MG1655
- 30oC, 48h
- Equipment: