Difference between revisions of "Part:BBa B0034:Experience"
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The ligation of part <partinfo>BBa_K190028</partinfo> behind the RBS was successful, confirmed by gel (correct vector size after digestion with EcoRI and PstI) and sequencing with VF2 primer. We used this part in combination with several genes for building our biobricks e.g. <partinfo>BBa_K190061</partinfo>. | The ligation of part <partinfo>BBa_K190028</partinfo> behind the RBS was successful, confirmed by gel (correct vector size after digestion with EcoRI and PstI) and sequencing with VF2 primer. We used this part in combination with several genes for building our biobricks e.g. <partinfo>BBa_K190061</partinfo>. | ||
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| + | <partinfo>BBa_B0034 AddReview 5</partinfo> | ||
| + | <I>maven</I> | ||
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| + | Defining efficiency for an RBS seems misleading, since RBS efficiency can only be considered in context of its upstream and downstream sequences as this [http://www.nature.com/nbt/journal/v27/n10/abs/nbt.1568.html|article] demonstrated. | ||
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Revision as of 12:44, 19 May 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_B0034
User Reviews
UNIQb66c4be8ed008e34-partinfo-00000000-QINU
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
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Aberdeen_Scotland 2009 |
Our miniprep, double digest and gel worked properly. We used this part in fusion PCR for building our biobrick BBa_K182102. The fusion PCR, cloning and sequence were all correct. |
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iGEM Groningen 2009 |
The ligation of part BBa_K190028 behind the RBS was successful, confirmed by gel (correct vector size after digestion with EcoRI and PstI) and sequencing with VF2 primer. We used this part in combination with several genes for building our biobricks e.g. BBa_K190061. |
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•••••
maven |
Defining efficiency for an RBS seems misleading, since RBS efficiency can only be considered in context of its upstream and downstream sequences as this [http://www.nature.com/nbt/journal/v27/n10/abs/nbt.1568.html|article] demonstrated. |
UNIQb66c4be8ed008e34-partinfo-00000008-QINU