Difference between revisions of "Part:BBa B0014:Experience"
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The plasmid containing part <partinfo>BBa_B0014</partinfo> was successfully transformed into ''E. coli'' TOP10 cells (confirmed by single and double digestion). The original plan was to use the terminator behind one of our own designed parts <partinfo>BBa_K190027</partinfo>, and in front of pArsR-RFP. The MBP-ArsR fusion protein was thought to have a regulating effect on the arsenic promotor pArsR in the same way as ArsR regulates the promotor. The terminator separated the two parts on the plasmid. The construct was not used due to time constraints, and not sequenced. | The plasmid containing part <partinfo>BBa_B0014</partinfo> was successfully transformed into ''E. coli'' TOP10 cells (confirmed by single and double digestion). The original plan was to use the terminator behind one of our own designed parts <partinfo>BBa_K190027</partinfo>, and in front of pArsR-RFP. The MBP-ArsR fusion protein was thought to have a regulating effect on the arsenic promotor pArsR in the same way as ArsR regulates the promotor. The terminator separated the two parts on the plasmid. The construct was not used due to time constraints, and not sequenced. | ||
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| + | <partinfo>BBa_B0014 AddReview 5</partinfo> | ||
| + | <I>iGEM LMU-Munich 2012</I> | ||
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| + | The plasmid containing part <partinfo>BBa_B0014</partinfo> was successfully sequenced and succesfully used as terminator for several constructs. We offer this part in pSB1C3: [https://parts.igem.org/Part:BBa_K823017 BBa_K823017] | ||
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Latest revision as of 16:54, 23 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_B0014
User Reviews
UNIQ1a1fee78f0304165-partinfo-00000000-QINU
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
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iGEM Groningen 2009 |
The plasmid containing part BBa_B0014 was successfully transformed into E. coli TOP10 cells (confirmed by single and double digestion). The original plan was to use the terminator behind one of our own designed parts BBa_K190027, and in front of pArsR-RFP. The MBP-ArsR fusion protein was thought to have a regulating effect on the arsenic promotor pArsR in the same way as ArsR regulates the promotor. The terminator separated the two parts on the plasmid. The construct was not used due to time constraints, and not sequenced. |
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•••••
iGEM LMU-Munich 2012 |
The plasmid containing part BBa_B0014 was successfully sequenced and succesfully used as terminator for several constructs. We offer this part in pSB1C3: BBa_K823017 |
UNIQ1a1fee78f0304165-partinfo-00000007-QINU