Difference between revisions of "Part:BBa K4897002"
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<partinfo>BBa_K4897002 short</partinfo>
 
<partinfo>BBa_K4897002 short</partinfo>
  
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BS DNA-322 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. Binding region is the key element in reacting with P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single strand DNA meanwhile the phi29 will generate double stranded DNA through amplification primers
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BS DNA-322 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element in reacting with the P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single-strand DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers
  
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===Usage and Biology===
 
===Usage and Biology===
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BS DNA-322 would bind the bacterial DNA of P. acne and show great sensitivity and specificity. It can stably bind to the specific bacterial DNA of P. acne and quantitatively indicate the amount of P. acne in the amplification result.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4897002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4897002 SequenceAndFeatures</partinfo>

Revision as of 10:48, 7 October 2023


BS DNA-322 (BS DNA 3.0) using in L-RCA for detecting P. acne

BS DNA-322 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element in reacting with the P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single-strand DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers

Usage and Biology

BS DNA-322 would bind the bacterial DNA of P. acne and show great sensitivity and specificity. It can stably bind to the specific bacterial DNA of P. acne and quantitatively indicate the amount of P. acne in the amplification result.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 161
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 161
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 161
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]