Difference between revisions of "Part:BBa K4634001"
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efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989). | efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989). | ||
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− | <span class='h3bb'> | + | ===Sequence and Features=== |
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+ | <partinfo>BBa_K4634002 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K4634002 parameters</partinfo> |
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Revision as of 04:23, 4 October 2023
pET28a(+) backbone
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5242
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 5248 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5242
Illegal BglII site found at 5130
Illegal XhoI site found at 22 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 5242
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 5242
Plasmid lacks a suffix.
Illegal XbaI site found at 5257
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 303
Illegal NgoMIV site found at 3350
Illegal NgoMIV site found at 3510
Illegal NgoMIV site found at 5098 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 2429