Difference between revisions of "Part:BBa K4839011"

 
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In order to prove our concept, we construct an expression cassette of GAL4 BS-UAS-GFP-pGK-mcherry. The GAL4 BS (GAL4 binding site) can bind with the GAL4 element and the UAS element can bind with the VP64 element. Once the SNIPR receptor recognize and target to the GPC3 receptor of Huh7 or THP-1 cancer cell, it will mediate downstream signal transduction and cut off the GAL4-VP64 factor. The GAL4-VP64 will bind to the GAL4 binding site and trigger the binding of VP64 and UAS element. The UAS promoter then activated by this transcriptional factor and start the transcription.  
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In order to prove our concept, we construct an expression cassette of GAL4 BS-UAS-GFP-pGK-mcherry. The GAL4 BS (GAL4 binding site) can bind with the GAL4 element and the UAS element can bind with the VP64 element. Once the SNIPR receptor recognize and target to the GPC3 receptor of Huh7 or THP-1 cancer cell, it will mediate downstream signal transduction and cut off the GAL4-VP64 factor. The GAL4-VP64 will bind to the GAL4 binding site and trigger the binding of VP64 and UAS element. The UAS promoter then activated by this transcriptional factor and start the transcription. (Figure1)
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<html><img src="https://static.igem.wiki/teams/4839/wiki/parts-files/9-1.png" width="650"</html>
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<p align="center">Figure1. The prove-of-concept of our SNIPR design</p>
  
 
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Revision as of 15:00, 3 October 2023


UAS (VP64 binding element)


In order to prove our concept, we construct an expression cassette of GAL4 BS-UAS-GFP-pGK-mcherry. The GAL4 BS (GAL4 binding site) can bind with the GAL4 element and the UAS element can bind with the VP64 element. Once the SNIPR receptor recognize and target to the GPC3 receptor of Huh7 or THP-1 cancer cell, it will mediate downstream signal transduction and cut off the GAL4-VP64 factor. The GAL4-VP64 will bind to the GAL4 binding site and trigger the binding of VP64 and UAS element. The UAS promoter then activated by this transcriptional factor and start the transcription. (Figure1)


Figure1. The prove-of-concept of our SNIPR design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]