Difference between revisions of "Part:BBa K4759025"
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(S)-4-amino-5-oxopentanoate <=> 5-aminolevulinate | (S)-4-amino-5-oxopentanoate <=> 5-aminolevulinate | ||
− | + | ===Design Notes=== | |
+ | Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria | ||
+ | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:05, 10 October 2023
hemL
the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.Glutamate-1-semialdehyde aminotransferase catalyzes the pyridoxal 5'-phosphate-dependent reaction which converts GSA to delta-aminolevulinate (ALA). (S)-4-amino-5-oxopentanoate <=> 5-aminolevulinate
Design Notes
Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1028
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1028
Illegal NheI site found at 738 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1028
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1028
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1028
Illegal AgeI site found at 388
Illegal AgeI site found at 721 - 1000COMPATIBLE WITH RFC[1000]