Difference between revisions of "Part:BBa K4759020"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | ===Design Notes=== | ||
+ | Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria | ||
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Revision as of 06:15, 10 October 2023
hemA
the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis. Forms part of the pathway for the biosynthesis of 5-aminolevulinate from glutamate, known as the C5 pathway, which is used in most eubacteria, and in all archaebacteria, algae and plants. (S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) <=> H(+) + L-glutamyl-tRNA(Glu) + NADPH
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 246
Illegal BglII site found at 524 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 178