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Revision as of 08:35, 2 October 2023


shuttle test contributed by Fudan iGEM 2023

Contents


Introduction

This composite part is designed to assess whether pRAP can function in cyanobacteria. It includes StayGold, stem-loop, Twister P1, and smURFP. When both red and green fluorescence can be expressed, it can be concluded that pRAP is functional in cyanobacteria. This provides a convenient expression system for genetic modification in cyanobacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1181
    Illegal BsaI.rc site found at 1201