Difference between revisions of "Part:BBa K4765129"
(→‎Introduction)
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===Introduction===
 
===Introduction===
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This compoiste part is a stem-loop-deleted version of [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120( ribozyme test: T7 leaky expression)], which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with [something] to assess the stem-loop's ability to prevent mRNA degradation.
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This compoiste part is a stem-loop-deleted version of [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120], which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120] to assess the stem-loop's ability to prevent mRNA degradation.
  
 
===Characterization===
 
===Characterization===

Revision as of 03:21, 8 October 2023


stem-loop test contributed by Fudan iGEM 2023


Introduction

This compoiste part is a stem-loop-deleted version of BBa_K4765120, which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with BBa_K4765120 to assess the stem-loop's ability to prevent mRNA degradation.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1389
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 700
    Illegal BsaI.rc site found at 720