Difference between revisions of "Part:BBa K4765013"
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===Introduction=== | ===Introduction=== | ||
| − | A D-Ala-D-Ala | + | A D-Ala-D-Ala ligase gene MysD converts Mycosporine-glycine(MG) tO MAA analogues like shinorine or porphyra-334 <ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 09:46, 3 October 2023
MysD codon optimized
Introduction
A D-Ala-D-Ala ligase gene MysD converts Mycosporine-glycine(MG) tO MAA analogues like shinorine or porphyra-334 [1].
Usage and Biology
We performed codon optimization on BBa_K4273013(NlmysD) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.
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| Figure1 The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr. 1 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1003
References
- ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.