Difference between revisions of "Part:BBa K4765013"
(→Introduction) |
(→Usage and Biology) |
||
| Line 9: | Line 9: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273013 BBa_K4273013 | + | We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273013 BBa_K4273013(NlmysD) ] specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''. |
{| | {| | ||
| <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/t-fudan-maa-pathway-wyj.png" alt="contributed by Fudan iGEM 2023"></html> | | <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/t-fudan-maa-pathway-wyj.png" alt="contributed by Fudan iGEM 2023"></html> | ||
| Line 25: | Line 25: | ||
<partinfo>BBa_K4765013 parameters</partinfo> | <partinfo>BBa_K4765013 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | |||
==References== | ==References== | ||
<references /> | <references /> | ||
Revision as of 07:05, 2 October 2023
MysD codon optimized
Introduction
A D-Ala-D-Ala ligaselike enzyme gene MysD converts Mycosporine-glycine(MG) tO MAA analogues like shinorine or porphyra-334 [1].
Usage and Biology
We performed codon optimization on BBa_K4273013(NlmysD) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.
|
| Figure1 The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr. 1 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1003
References
- ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.