Difference between revisions of "Part:BBa K4645001:Design"
Ruixuan Zhu (Talk | contribs) |
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===Design Notes=== | ===Design Notes=== | ||
| − | On the basis of the expression of functional target proteins, 6*His tag is added, which can help us better obtain high-purity target proteins. | + | On the basis of the expression of functional target proteins, 6*His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N terminal of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization. |
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===Source=== | ===Source=== | ||
| − | Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies.The amino acid sequence of one of the most effective monoclonal antibodies was obtained through literature review. | + | Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies.The amino acid sequence of one of the most effective monoclonal antibodies REGN 1908 was obtained through literature review. |
===References=== | ===References=== | ||
Revision as of 15:07, 5 October 2023
NeuA: scFv ( single-chain fragment variable）that inhibits the immune response to the feline
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 315
Illegal XhoI site found at 693 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
On the basis of the expression of functional target proteins, 6*His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N terminal of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.
Source
Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies.The amino acid sequence of one of the most effective monoclonal antibodies REGN 1908 was obtained through literature review.