Difference between revisions of "Part:BBa K4765122"
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===Introduction=== | ===Introduction=== | ||
| − | This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes ''E. coli pgmA'' on the downstream site and ''E. coli galU'' on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 | + | This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes ''E. coli pgmA'' on the downstream site and ''E. coli galU'' on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 BBa_K3331001]and [https://parts.igem.org/Part:BBa_K3331002 BBa_K3331002]. The following mScarlet is utilized to detection E. coli with adhesion ability. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 04:14, 2 October 2023
ribozyme connected: pgmA + galU + mScarlet
Introduction
This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes E. coli pgmA on the downstream site and E. coli galU on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in BBa_K3331001and BBa_K3331002. The following mScarlet is utilized to detection E. coli with adhesion ability.
Usage and Biology
We use this part to improve EPS production of E. coli and assessment of EPS’s adhesion ability.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 3285
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]