Difference between revisions of "Part:BBa K4726002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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In order to clone this genetic construct onto our expression vector (pET-3a) we added NdeI site in 5' and BamHI site in 3' of the gene. We added TATATA sequences before the NdeI site and after the BamHI site to assist with the restriction digestion.
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===Source===
 
===Source===

Revision as of 00:40, 10 October 2023


α-galactosidase GH110b_Bacteroides fragilis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1213
    Illegal EcoRI site found at 1714
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1213
    Illegal EcoRI site found at 1714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1213
    Illegal EcoRI site found at 1714
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1213
    Illegal EcoRI site found at 1714
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1213
    Illegal EcoRI site found at 1714
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to clone this genetic construct onto our expression vector (pET-3a) we added NdeI site in 5' and BamHI site in 3' of the gene. We added TATATA sequences before the NdeI site and after the BamHI site to assist with the restriction digestion.

Source

jbunn

References