Difference between revisions of "Part:BBa K4726001"

 
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<partinfo>BBa_K4726001 short</partinfo>
 
<partinfo>BBa_K4726001 short</partinfo>
  
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This encodes a α-D-galactosamine galactosaminidase from <i>Flavonifractor plautii</i> originating from the human gut microbiota.
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This enzyme removes the acetyl moiety of N-acetyl-α-D-galactosamine (A antigen). This enzyme works in pair with P0DTR4 to fully remove the A antigen. P0DTR5 is not active towards A antigen without prior reaction with P0DTR4.
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The expression of this enzyme led to significant proteotoxicity shown by an impaired growth of <i>Escherichia coli</i> BL21 (DE3). We thus recommended to use Escherichia coli Rosetta (DE3) pLysS which helps stabilizing pET recombinants encoding proteins.
  
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
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<partinfo>BBa_K4726001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4726000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4726001 parameters</partinfo>
 
<partinfo>BBa_K4726001 parameters</partinfo>
 
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===References===
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[1] Rahfeld P, Sim L, Moon H, Constantinescu I, Morgan-Lang C, Hallam SJ, Kizhakkedathu JN, Withers SG. An enzymatic pathway in the human gut microbiome that converts A to universal O type blood. Nat Microbiol. 2019 Sep;4(9):1475-1485. doi: 10.1038/s41564-019-0469-7. Epub 2019 Jun 10. PMID: 31182795.

Revision as of 00:29, 10 October 2023


α-D-galactosamine galactosaminidase_Flavonifractor plautii

This encodes a α-D-galactosamine galactosaminidase from Flavonifractor plautii originating from the human gut microbiota.

This enzyme removes the acetyl moiety of N-acetyl-α-D-galactosamine (A antigen). This enzyme works in pair with P0DTR4 to fully remove the A antigen. P0DTR5 is not active towards A antigen without prior reaction with P0DTR4.

The expression of this enzyme led to significant proteotoxicity shown by an impaired growth of Escherichia coli BL21 (DE3). We thus recommended to use Escherichia coli Rosetta (DE3) pLysS which helps stabilizing pET recombinants encoding proteins.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1894
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Rahfeld P, Sim L, Moon H, Constantinescu I, Morgan-Lang C, Hallam SJ, Kizhakkedathu JN, Withers SG. An enzymatic pathway in the human gut microbiome that converts A to universal O type blood. Nat Microbiol. 2019 Sep;4(9):1475-1485. doi: 10.1038/s41564-019-0469-7. Epub 2019 Jun 10. PMID: 31182795.