Difference between revisions of "Part:BBa K4765121"
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===Introduction===
 
===Introduction===
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This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes ''E. coli'' galU on the upstream site and ''E. coli'' pgmA on the downstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 E.coli galU]and [https://parts.igem.org/Part:BBa_K3331002 E.coli pgmA]. The following mScarlet is utilized to detection E. coli with adhesion ability.
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This composite part is designed for the production of exopolysaccharide (EPS), and detection of EPS’s adhesion ability. It includes ''E. coli'' galU on the upstream site and ''E. coli'' pgmA on the downstream site, two essential enzymes in EPS biosynthesis pathway <ref>需要参考文献</ref>. These two enzymes were tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 K3331001]and [https://parts.igem.org/Part:BBa_K3331002 K3331002]. The following mScarlet is utilized to detection ''E. coli'' with adhesion ability.
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===Usage and Biology===
 
===Usage and Biology===
 
We use this part to improve EPS production of ''E. coli'' and assessment of EPS’s adhesion ability.
 
We use this part to improve EPS production of ''E. coli'' and assessment of EPS’s adhesion ability.

Revision as of 00:32, 2 October 2023


ribozyme connected: galU + pgmA + mScarlet contributed by Fudan iGEM 2023


Introduction

This composite part is designed for the production of exopolysaccharide (EPS), and detection of EPS’s adhesion ability. It includes E. coli galU on the upstream site and E. coli pgmA on the downstream site, two essential enzymes in EPS biosynthesis pathway [1]. These two enzymes were tested by iGEM20_XJTU-China in K3331001and K3331002. The following mScarlet is utilized to detection E. coli with adhesion ability.

Usage and Biology

We use this part to improve EPS production of E. coli and assessment of EPS’s adhesion ability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3285
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ 需要参考文献