Difference between revisions of "Part:BBa K4765121"
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===Introduction=== | ===Introduction=== | ||
| − | This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes | + | This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes ''E. coli'' galU on the upstream site and ''E. coli'' pgmA on the downstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in [https://parts.igem.org/Part:BBa_K3331001 E.coli galU]and [https://parts.igem.org/Part:BBa_K3331002 E.coli pgmA]. The following mScarlet is utilized to detection E. coli with adhesion ability. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We use this part to improve EPS production of | + | We use this part to improve EPS production of ''E. coli'' and assessment of EPS’s adhesion ability. |
===Characterization=== | ===Characterization=== | ||
Revision as of 18:00, 1 October 2023
ribozyme connected: galU + pgmA + mScarlet
Introduction
This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes E. coli galU on the upstream site and E. coli pgmA on the downstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in E.coli galUand E.coli pgmA. The following mScarlet is utilized to detection E. coli with adhesion ability.
Usage and Biology
We use this part to improve EPS production of E. coli and assessment of EPS’s adhesion ability.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 3285
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]