Difference between revisions of "Part:BBa K4765117"
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__TOC__
  
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ribozyme+RBS+CDS+stem-loop module: H. ex mtSSB+SAHS 33020
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===Introduction===
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This composite part is composed of [https://parts.igem.org/Part:BBa_K4765112 ribozyme+T7_RBS+SAHS 33020+stem-loop] and [https://parts.igem.org/Part:BBa_K4765112 ribozyme+T7_RBS+''H. ex'' mtSSB+stem-loop].
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===Usage and Biology===
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We introduced this part into ''E. coli'', enabling the heterologous expression of both mtSSB and TDP proteins, to assess the combined desiccation resistance capability of these two proteins.
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===Characterization===
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Revision as of 15:19, 1 October 2023


ribozyme connected: H. ex mtSSB + SAHS 33020

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of ribozyme+T7_RBS+SAHS 33020+stem-loop and ribozyme+T7_RBS+H. ex mtSSB+stem-loop.

Usage and Biology

We introduced this part into E. coli, enabling the heterologous expression of both mtSSB and TDP proteins, to assess the combined desiccation resistance capability of these two proteins.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 880
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409