Difference between revisions of "Part:BBa K4645000:Design"

 
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===Design Notes===
 
===Design Notes===
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On the basis of the expression of functional target proteins, 6*His tag and Flag tag are added, which can help us better obtain high-purity target proteins and do subsequent experimental verification.  
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On the basis of the expression of functional target proteins, His tag and Flag tag are added, which can help us better obtain high-purity target proteins and do subsequent experimental verification.  
  
  
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===Source===
 
===Source===
  
−
Fel d 1 is a protein consisting of two amino acid chains encoded in the genome of domestic feiline. FelD is a recombinant protein formed by fusing 93 amino acids from chain 2 and 70 amino acids from chain 1 of Fel d 1, and with a 6*His tag and a Flag tag at the C-terminal.
+
Fel d 1 is a protein consisting of two amino acid chains encoded in the genome of domestic feiline. FelD is a recombinant protein formed by fusing 93 amino acids from chain 2 and 70 amino acids from chain 1 of Fel d 1, and with a His tag and a Flag tag at the C-terminus.
  
 
===References===
 
===References===

Revision as of 00:22, 12 October 2023


FelD: Felis domesticus allergen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the basis of the expression of functional target proteins, His tag and Flag tag are added, which can help us better obtain high-purity target proteins and do subsequent experimental verification.


Source

Fel d 1 is a protein consisting of two amino acid chains encoded in the genome of domestic feiline. FelD is a recombinant protein formed by fusing 93 amino acids from chain 2 and 70 amino acids from chain 1 of Fel d 1, and with a His tag and a Flag tag at the C-terminus.

References