Difference between revisions of "Part:BBa K4765022"
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===Introduction=== | ===Introduction=== | ||
| − | mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)<ref>Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., & Gadella, T. W., Jr (2017). mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature methods, 14(1), 53–56. https://doi.org/10.1038/nmeth.4074.</ref> | + | mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)<ref>Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., & Gadella, T. W., Jr (2017). mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature methods, 14(1), 53–56. https://doi.org/10.1038/nmeth.4074.</ref>. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
We constructed mScarlet into the composite [https://parts.igem.org/Part:BBa_K4765119 ribozyme measurement module: twister ribozyme (constitutive expression)] and [https://parts.igem.org/Part:BBa_K4765120 ribozyme measurement module: twister ribozyme (leakage expression)] to test the effiency of twister ribozyme and stem-loop. | We constructed mScarlet into the composite [https://parts.igem.org/Part:BBa_K4765119 ribozyme measurement module: twister ribozyme (constitutive expression)] and [https://parts.igem.org/Part:BBa_K4765120 ribozyme measurement module: twister ribozyme (leakage expression)] to test the effiency of twister ribozyme and stem-loop. | ||
Revision as of 13:34, 1 October 2023
mScarlet
Introduction
mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)[1].
Usage and Biology
We constructed mScarlet into the composite ribozyme measurement module: twister ribozyme (constitutive expression) and ribozyme measurement module: twister ribozyme (leakage expression) to test the effiency of twister ribozyme and stem-loop.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 512
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
=Reference
- ↑ Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., & Gadella, T. W., Jr (2017). mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature methods, 14(1), 53–56. https://doi.org/10.1038/nmeth.4074.