Difference between revisions of "Part:BBa K4765002"
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===Introduction=== | ===Introduction=== | ||
| − | Ag1 is a corresponding antigen of [https://parts.igem.org/Part:BBa_K4765003 Nb1]<ref>Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041</ref>. The interaction between Ag-Nb can mediate specific adhesion of ''E.coli''. | + | Ag1 is a corresponding antigen of [https://parts.igem.org/Part:BBa_K4765003 Nb1]<ref>Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. ''Cell, 174''(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041</ref>. The interaction between Ag-Nb can mediate specific adhesion of ''E.coli''. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
The combination of Ag1's single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein. | The combination of Ag1's single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein. | ||
Revision as of 14:48, 1 October 2023
Ag1, Akt3PH from 10.1016/j.cell.2018.06.041
Introduction
Ag1 is a corresponding antigen of Nb1[1]. The interaction between Ag-Nb can mediate specific adhesion of E.coli.
Usage and Biology
The combination of Ag1's single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 340
Reference
- ↑ Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041