Difference between revisions of "Part:BBa K4765102"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Similar to [https://parts.igem.org/Part:BBa_K4765104 ribozyme+strong RBS+intimin-Nb2 fusion+stem-loop], we replaced Nb2 with Nb1 in order to interact with [https://parts.igem.org/Part:BBa_K4765101 ribozyme+strong RBS+intimin-Ag1 fusion+stem-loop] to create a biofilm. | + | Similar to [https://parts.igem.org/Part:BBa_K4765104 BBa_K4765104(ribozyme+strong RBS+intimin-Nb2 fusion+stem-loop)], we replaced Nb2 with Nb1 in order to interact with [https://parts.igem.org/Part:BBa_K4765101 BBa_K4765101(ribozyme+strong RBS+intimin-Ag1 fusion+stem-loop)] to create a biofilm. |
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Revision as of 07:18, 2 October 2023
Twister P1 + T7_RBS + intimin-Nb1 fusion + stem-loop
Contents
Usage and Biology
Similar to BBa_K4765104(ribozyme+strong RBS+intimin-Nb2 fusion+stem-loop), we replaced Nb2 with Nb1 in order to interact with BBa_K4765101(ribozyme+strong RBS+intimin-Ag1 fusion+stem-loop) to create a biofilm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2075
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1305