Difference between revisions of "Part:BBa K4765118"
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===Introduction=== | ===Introduction=== | ||
| − | Mycosporine-like amino acids (MAAs), act as a sunscreen for the biofilm. This composite part contains five enyzmes in the MAA biosynthesis pathway. All the enzymes are constructed into ribozyme-assisted polycistronic co-expression system：pRAP. | + | Mycosporine-like amino acids (MAAs), act as a sunscreen for the biofilm. This composite part contains five enyzmes in the MAA biosynthesis pathway. All the enzymes are constructed into ribozyme-assisted polycistronic co-expression system：pRAP. |
| + | {| | ||
| + | | <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/t-fudan-maa-pathway-wyj.png" alt="contributed by Fudan iGEM 2023"></html> | ||
| + | |- | ||
| + | | '''Figure1 The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr''' | ||
| + | |} | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Biosynthetic route of MAA initiates with the generation of 4-deoxygadusol (4-DG) from sedoheptulose 7-phosphate, an intermediate within the pentose phosphate pathway. This process is catalyzed by two enzymes: a dimethyl 4-degadusol synthase (DDGS; MysA) and an Omethyltrans-ferase (O-MT; MysB). Subsequently, 4-DG undergoes a transformation into mycosporine-glycine(MG) through an ATP-grasp enzyme MysC, which introduces an amino acid moiety, primarily L-Gly. MAA analogues such as shinorine or porphyra-334 are further derived from MG by the D-Ala-D-Ala ligase-like enzyme MysD. In the final step, the biosynthesis is completed with a nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase MysH, leading to the production of palythines. | Biosynthetic route of MAA initiates with the generation of 4-deoxygadusol (4-DG) from sedoheptulose 7-phosphate, an intermediate within the pentose phosphate pathway. This process is catalyzed by two enzymes: a dimethyl 4-degadusol synthase (DDGS; MysA) and an Omethyltrans-ferase (O-MT; MysB). Subsequently, 4-DG undergoes a transformation into mycosporine-glycine(MG) through an ATP-grasp enzyme MysC, which introduces an amino acid moiety, primarily L-Gly. MAA analogues such as shinorine or porphyra-334 are further derived from MG by the D-Ala-D-Ala ligase-like enzyme MysD. In the final step, the biosynthesis is completed with a nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase MysH, leading to the production of palythines. | ||
Revision as of 05:47, 11 October 2023
ribozyme connected: MysABCDH
Introduction
Mycosporine-like amino acids (MAAs), act as a sunscreen for the biofilm. This composite part contains five enyzmes in the MAA biosynthesis pathway. All the enzymes are constructed into ribozyme-assisted polycistronic co-expression system：pRAP.
|
| Figure1 The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr |
Usage and Biology
Biosynthetic route of MAA initiates with the generation of 4-deoxygadusol (4-DG) from sedoheptulose 7-phosphate, an intermediate within the pentose phosphate pathway. This process is catalyzed by two enzymes: a dimethyl 4-degadusol synthase (DDGS; MysA) and an Omethyltrans-ferase (O-MT; MysB). Subsequently, 4-DG undergoes a transformation into mycosporine-glycine(MG) through an ATP-grasp enzyme MysC, which introduces an amino acid moiety, primarily L-Gly. MAA analogues such as shinorine or porphyra-334 are further derived from MG by the D-Ala-D-Ala ligase-like enzyme MysD. In the final step, the biosynthesis is completed with a nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase MysH, leading to the production of palythines.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1272
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1064
Illegal BsaI.rc site found at 2666
Illegal BsaI.rc site found at 3785
Illegal BsaI.rc site found at 5714