Difference between revisions of "Part:BBa K4765116"
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===Usage and Biology===
 
===Usage and Biology===
 
We constructed XRCC1 into pRAP to endow ''E. coli'' with anti-UV capability.
 
We constructed XRCC1 into pRAP to endow ''E. coli'' with anti-UV capability.
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===Characterization===
 
  
  
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<partinfo>BBa_K4765116 parameters</partinfo>
 
<partinfo>BBa_K4765116 parameters</partinfo>
 
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==Reference==
 

Revision as of 15:52, 12 October 2023


Twister P1 + T7_RBS + XRCC1 + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of XRCC1 and ribozyme-assisted polycistronic co-expression system：pRAP. XRCC1 is a vital protein in DNA repair, particularly for single-strand breaks caused by radiation and alkylating agents.

Usage and Biology

We constructed XRCC1 into pRAP to endow E. coli with anti-UV capability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 746
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1796