Difference between revisions of "Part:BBa K4604014"
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This BioBrick can be used to produce Adenosylcobalamin (vitamin B12) in E. coli when supplemented with cobinamide.
 
This BioBrick can be used to produce Adenosylcobalamin (vitamin B12) in E. coli when supplemented with cobinamide.
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The tet promoter used in this version of the plasmid is leaky, a modified version of it is BBa_K4604015, piG_01b.
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The tet promoter used in this version of the plasmid is a modified version of BBa_K4604015, piG_01a.
  
 
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Revision as of 10:18, 1 October 2023


piG_01a falsch

piG_01a is a plasmid consisting of the tet promoter/repressor, a functional bluB gene and the rrnB terminator. The backbone we used in the experiments is pGGAselect.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1618