Difference between revisions of "Part:BBa K260003"

 
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<partinfo>BBa_K260003 short</partinfo>
 
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The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.  
 
The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.  
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This plasmid backbone is available with part [[Part:BBa_K260017|BBa_K260017]], with which it forms a functional unit: [L-rhamnose] -> [FLP] -> RFP
  
 
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Revision as of 17:56, 17 October 2009

pRhaFlp. [L-rhamnose] -> FLP(F70L)

This plasmid is based on pSC101 and contains the rhamnose operon (rhaR and rhaS genes) as well as the F70L variant of FLP recombinase under rhamnose-promoter control. Therefore L-rhamnose can be used to induce FLP(F70L) expression.

The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.

This plasmid backbone is available with part BBa_K260017, with which it forms a functional unit: [L-rhamnose] -> [FLP] -> RFP

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3471
    Illegal EcoRI site found at 4272
    Illegal SpeI site found at 1125
    Illegal SpeI site found at 3153
    Illegal PstI site found at 2466
    Illegal PstI site found at 3992
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3471
    Illegal EcoRI site found at 4272
    Illegal EcoRI site found at 6324
    Illegal NheI site found at 102
    Illegal NheI site found at 5981
    Illegal SpeI site found at 2
    Illegal SpeI site found at 1125
    Illegal SpeI site found at 3153
    Illegal PstI site found at 16
    Illegal PstI site found at 2466
    Illegal PstI site found at 3992
    Illegal NotI site found at 9
    Illegal NotI site found at 6330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3471
    Illegal EcoRI site found at 4272
    Illegal EcoRI site found at 6324
    Illegal BglII site found at 2679
    Illegal BglII site found at 4952
    Illegal XhoI site found at 61
    Illegal XhoI site found at 6318
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 6324
    Illegal suffix found at 2
    Illegal EcoRI site found at 3471
    Illegal EcoRI site found at 4272
    Illegal SpeI site found at 1125
    Illegal SpeI site found at 3153
    Illegal PstI site found at 2466
    Illegal PstI site found at 3992
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 6324
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3471
    Illegal EcoRI site found at 4272
    Illegal XbaI site found at 6339
    Illegal SpeI site found at 2
    Illegal SpeI site found at 1125
    Illegal SpeI site found at 3153
    Illegal PstI site found at 16
    Illegal PstI site found at 2466
    Illegal PstI site found at 3992
    Illegal NgoMIV site found at 2015
    Illegal AgeI site found at 4684
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 1955
    Illegal SapI site found at 2165