Difference between revisions of "Part:BBa K4885989"

Latest revision as of 05:59, 10 October 2023

100

Sequence and Features

Assembly Compatibility:

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 <partinfo>BBa_K4885989 short</partinfo>
-This part is responsible for the expression of adhE2 gene with Pthl promotor and Dac gene with Pcatl promotor. adhE2 gene is derived from Clostridium acetobutylicum. Dac gene is derived from Clostridium tyrobutyricum (C. tyrobutyricum) L319. It consists of Pthl sequence (BBa_K3443002), strong ribosomal binding site (RBS) sequence  (BBa_K103015),  adhE2 sequence(BBa_K1462060), Dac gene sequence (BBa_K4885000) and terminator sequence  (BBa_K3585002). adhE2 gene codes for the alcohol/aldehyde bifunctional dehydrogenase. Its role is to convert acyl-CoA to aldehyde then to alcohol in two reductive steps using NADH as cofactor. Dac gene codes a NAD-dependent deacetylase which is an enzyme removing acetyl groups from the N-terminal of protein substrates and weakening the N-terminal acetylation of proteins. The enzyme is responsible for the removal of N-terminal acetylation modification in the protein post-translational modification system.
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 <partinfo>BBa_K4885989 SequenceAndFeatures</partinfo>
-==Results==
-===（1）Plasmid construction===
-===（2）Construction of C. tyrobutyricum L319 with pMTL-Pthl-adhE2-Dac===
-<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K4885989 parameters</partinfo>
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