Difference between revisions of "Part:BBa K4765012"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4765012 short</partinfo> | <partinfo>BBa_K4765012 short</partinfo> | ||
| + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> | ||
__TOC__ | __TOC__ | ||
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===Introduction=== | ===Introduction=== | ||
An ATP-grasp enzyme MysC introduces L-Glycine to 4-DG at C3, and produces mycosporineglysine (MG)<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>. | An ATP-grasp enzyme MysC introduces L-Glycine to 4-DG at C3, and produces mycosporineglysine (MG)<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>. | ||
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| + | | <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/t-fudan-maa-pathway-wyj.png" alt="contributed by Fudan iGEM 2023"></html> | ||
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| + | | '''Figure1 The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr. 1''' | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:11, 1 October 2023
MysC codon optimized
Introduction
An ATP-grasp enzyme MysC introduces L-Glycine to 4-DG at C3, and produces mycosporineglysine (MG)[1].
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| Figure1 The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr. 1 |
Usage and Biology
We performed codon optimization on BBa_K4273008 (NpR5598) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysC catalyzes the third reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 736
References
- ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.