Difference between revisions of "Part:BBa K4642034"
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This composite part is used for testing the presence of hsa-miR-320b, hsa-miR-320a and hsa-miR-4640. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642028 and the anti-miRNAs BBa_K4642029 and BBa_K4642030. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is mCerulean BBa_K3075004. This fluorescence produced can be detected with a luminometer to affirm a positive test. | This composite part is used for testing the presence of hsa-miR-320b, hsa-miR-320a and hsa-miR-4640. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642028 and the anti-miRNAs BBa_K4642029 and BBa_K4642030. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is mCerulean BBa_K3075004. This fluorescence produced can be detected with a luminometer to affirm a positive test. | ||
Latest revision as of 14:58, 11 October 2023
T7 / Gen4 320b-320a-4640 Double AND-Gate / mCerulean / Double Terminator / T7 / Gen4 320b-320a-4640
This composite part is used for testing the presence of hsa-miR-320b, hsa-miR-320a and hsa-miR-4640. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642028 and the anti-miRNAs BBa_K4642029 and BBa_K4642030. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is mCerulean BBa_K3075004. This fluorescence produced can be detected with a luminometer to affirm a positive test.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 216
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]