Difference between revisions of "Part:BBa K4642029:Design"

Latest revision as of 18:22, 28 September 2023

Gen4 320b-320a-4640 Double AND-Gate Toehold Switch Anti-miRNA 4640-320a

Assembly Compatibility:

We had to ensure our anti-miRNA alone did not bind to the toehold switch.

Custom designed in-silico using NUPACK API.

Revision as of 18:20, 28 September 2023 (view source) Registry (Talk \| contribs)		Latest revision as of 18:22, 28 September 2023 (view source) RYapa (Talk \| contribs)
Line 7:		Line 7:

	===Design Notes===		===Design Notes===
−	~~A riboregulator that activates gene expression must switch from a secondary structure that prevents translation~~ to ~~a configuration that promotes translation upon binding of a cognate transacting RNA. Although the Shine~~-~~Dalgarno sequence is an important factor in determining~~ the efficiency of translation from a given RNA, studies have found that secondary structure in regions nearby the start codon also plays a critical role. Furthermore, genome-wide analyses have revealed strong biases toward low secondary structures around the start codon of mRNAs from a panel of hundreds of bacterial genomes. Thus, we ensured that toehold ~~switches sequestered the region around the start codon to repress translation, rather than binding to either the RBS or the start codon~~.	+	We had to ensure our anti-miRNA alone did not bind to the toehold switch.