Difference between revisions of "Part:BBa K4642027"
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This composite part is used for testing the presence of hsa-miR-373, hsa-miR-21 and hsa-miR-24. It has a T7 promoter for expression of functional RNA, BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642021 and the anti-miRNAs BBa_K4642022 and BBa_K4642023 Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is Venus, BBa_K1976002. This fluorescence produced can be detected with a luminometer to affirm a positive test. | This composite part is used for testing the presence of hsa-miR-373, hsa-miR-21 and hsa-miR-24. It has a T7 promoter for expression of functional RNA, BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642021 and the anti-miRNAs BBa_K4642022 and BBa_K4642023 Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is Venus, BBa_K1976002. This fluorescence produced can be detected with a luminometer to affirm a positive test. | ||
Latest revision as of 14:56, 11 October 2023
T7 / Gen4 373-21-24 Double AND-Gate / Venus / Double Terminator / T7 / Gen4 373-21-24 Double AND-Gat
This composite part is used for testing the presence of hsa-miR-373, hsa-miR-21 and hsa-miR-24. It has a T7 promoter for expression of functional RNA, BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642021 and the anti-miRNAs BBa_K4642022 and BBa_K4642023 Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is Venus, BBa_K1976002. This fluorescence produced can be detected with a luminometer to affirm a positive test.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]