Difference between revisions of "Part:BBa K4887001"
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The encoding gene of granule-bound starch synthase I (GBSSI) in sweet potato (Ipomoea batatas). It is one of the starch biosynthetic pathway genes on the starch biosynthetic pathway.
 
The encoding gene of granule-bound starch synthase I (GBSSI) in sweet potato (Ipomoea batatas). It is one of the starch biosynthetic pathway genes on the starch biosynthetic pathway.
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The selected sgRNA is located 70 bp downstream of the start codon, with a total sequence length of 27 bp (Fig. 1). sgRNA oligos were obtained by annealing with the designed primers following:
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Oligo 1 (GBSSI-sgRNA1): 5’-GTGGGGTTGGGTCAATTAGCCCTGAGGAGC-3'
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Oligo 2 (GBSSI-sgRNA2): 5’-AACTGGTGGACTTGGAGATGTTCTTGGAGG-3’
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 09:39, 6 October 2023


IbGBSSI

The encoding gene of granule-bound starch synthase I (GBSSI) in sweet potato (Ipomoea batatas). It is one of the starch biosynthetic pathway genes on the starch biosynthetic pathway.

The selected sgRNA is located 70 bp downstream of the start codon, with a total sequence length of 27 bp (Fig. 1). sgRNA oligos were obtained by annealing with the designed primers following: Oligo 1 (GBSSI-sgRNA1): 5’-GTGGGGTTGGGTCAATTAGCCCTGAGGAGC-3' Oligo 2 (GBSSI-sgRNA2): 5’-AACTGGTGGACTTGGAGATGTTCTTGGAGG-3’

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1459
    Illegal BsaI.rc site found at 123
    Illegal SapI site found at 1156
    Illegal SapI.rc site found at 1225