Difference between revisions of "Part:BBa K4593002:Design"

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===Design Notes===
 
===Design Notes===
−
The sequence does not contain a start codon and must be cloned to vectors used for protein purification (such as pET-28 in our project) before being used.
+
N/A
  
 
===Source===
 
===Source===

Revision as of 15:13, 3 October 2023


ClyC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 681
    Illegal AgeI site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A

Source

Hybrid endolysin from domains of 12 natural staphylococcal endolysins.

References

Lee, Chanyoung, et al. “Development of Advanced Chimeric Endolysin to Control Multidrug-Resistant Staphylococcus Aureus through Domain Shuffling.” ACS Infectious Diseases, vol. 7, no. 8, 28 May 2021, pp. 2081–2092. https://doi.org/10.1021/acsinfecdis.0c00812.