Difference between revisions of "Part:BBa K4824000:Experience"
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===Applications of BBa_K4824000===
 
===Applications of BBa_K4824000===
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=Introduction=
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Bioparts are the basic building blocks of the iGEM project. Here, we constructed a catalytic lignan glycoside (BBa_K4824000) biopart (Figure 1). Based on the reported transcriptomic data of I. indigotica, the sequences of genes with glycosyltransferase functions were screened and a phylogenetic tree analysis was conducted with the functionally characterized lignan UGTs, and UGT72B2 was selected as the candidate, which the main component of the biopart. We then constructed expression vectors using homologous recombination and ultimately verified protein function.
  
 
===User Reviews===
 
===User Reviews===
 
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=Introduction=
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Bioparts are the basic building blocks of the iGEM project. Here, we constructed a catalytic lignan glycoside (BBa_K4824000) biopart (Figure 1). Based on the reported transcriptomic data of I. indigotica, the sequences of genes with glycosyltransferase functions were screened and a phylogenetic tree analysis was conducted with the functionally characterized lignan UGTs, and UGT72B2 was selected as the candidate, which the main component of the biopart. We then constructed expression vectors using homologous recombination and ultimately verified protein function.
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Revision as of 06:35, 25 September 2023


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Please enter how you used this part and how it worked out.

Applications of BBa_K4824000

Introduction

Bioparts are the basic building blocks of the iGEM project. Here, we constructed a catalytic lignan glycoside (BBa_K4824000) biopart (Figure 1). Based on the reported transcriptomic data of I. indigotica, the sequences of genes with glycosyltransferase functions were screened and a phylogenetic tree analysis was conducted with the functionally characterized lignan UGTs, and UGT72B2 was selected as the candidate, which the main component of the biopart. We then constructed expression vectors using homologous recombination and ultimately verified protein function.

User Reviews

UNIQ4cce18e56ea77bdc-partinfo-00000000-QINU UNIQ4cce18e56ea77bdc-partinfo-00000001-QINU