Difference between revisions of "Part:BBa K4586018"

Revision as of 04:24, 25 September 2023

CD 8 alpha signal

Description

CD8 alpha is signal peptide translocate the conjugated protein to the cellular membrane.

Literature Characterization

The study created a reporter construct by joining the C-terminus of CD63, one of the most used exosome markers, to nanoluc (nluc), a tiny and potent bioluminescence reporter10. After progressive centrifugation to eliminate masking signals12, luminescence in the cell-culture supernatant was measured. This reporter gene was co-transfected with plasmids expressing potential candidates for exosome production augmentation.

Poly(A) RNA samples from each cell line were separated by gel electrophoresis, transferred to a nitrocellulose membrane, and hybridized with 32P-labeled cDNA probes specific for MC-CPA or CD8amp;. The membranes were then reprobed with a 32P-labeled p-actin cDNA probe to verify that equal amounts of RNA had been loaded in each lane. The results showed that MC-CPA mRNA was expressed at a higher level in MW9.11-3 cells than in MC/9.IL-4 cells, while CDalpha mRNA was expressed at a similar level in both cell lines. These findings suggest that MC-CPA and CDalpha may play different roles in the development and differentiation of these two cell

References

Hara, T., Harada, N., Mitsui, H., Miura, T., Ishizaka, T., & Miyajima, A. (1994). Characterization of cell phenotype by a novel cDNA library subtraction system: expression of CD8 alpha in a mast cell-derived interleukin-4-dependent cell line.

Sequence and Features