Difference between revisions of "Part:BBa K4585000"
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Revision as of 16:32, 28 September 2023
GnRH-C
A protein of truncated gonadotropin-releasing hormone.
Insulin
We have proved that Insulin can be processed and secreted outside the cell in 293T cells.
1 diagram
Fig.1 The model diagram of 9XUAS-Insulin
2.Experiment
2.1 Method
In the experiment, we used an ELIAS kit specially designed to detect human mature insulin to detect cell eliquidation.
2.2 Results
Insulin can be processed and secreted outside the cell in 293T cells.
Fig.2 Standard curve of absorbance and insulin concentration
Fig.3 Insulin concentration in supernatant after blue light irradiation and dark treatment respectively
Fig.4 Insulin concentration in supernatant (excluding insulin in medium) after blue light irradiation and dark treatment respectively
3.Caution
Insulin is not suitable for long time preservation under the best culture temperature of cells (37℃). In the mean time, cells would also consume some of the expressed insulin. So the best tst time for Insulin is approximately 24 hours after transfection, it is relatively shorter than the test interval of LUC.
Reference：
[1]Mingqi Xie, Haifeng Ye, Hui Wang.β-cell-mimetic designer cells provide closed-loop glycemic control[J].Science.2016 Dec 9;354(6317):1296-1301.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]