Difference between revisions of "Part:BBa K4601041"

 
 
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<partinfo>BBa_K4601041 short</partinfo>
 
<partinfo>BBa_K4601041 short</partinfo>
  
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This part is AmpR, an ''E. coli'' gene coding for a β-lactamase enzyme. It is a derivative of the TEM-1 β-lactamase (Uniprot P62593) with two mutations V82I and V182A. In addition, compared to the sequence found in pSB1A3 from which it was derived by PCR amplification, two synonymous mutations Y44 (TAT->TAC) and S240 (TCC->TCG) were detected.
  
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===Usage and Biology===
 
===Usage and Biology===
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AmpR is a protein associated with the resistance mechanism against the large family of β-lactams, antibiotics which include the well known penicillin and ampicillin.
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β-Lactamases act by hydrolysing the β-lactam ring (Figure 1), thus inactivating the antibiotic.
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[[File: T--Evry_Paris-Saclay--beta-lactam_ring_hydrolysis.png|center|300px]]
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Figure 1. The mode of action of β-lactamases: hydrolysis of the β-lactam ring.
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Fortunately, among all the subgroups of β-lactams (table 1), some have less affinity with the β-lactamase(s) and overcome this resistance.
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{| class="wikitable centre"
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!colspan="9"|Table 1. β-lactam groups and sub-groups, classified based on their core ring structures.
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|-
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|penams
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|carbapenams   
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|clavams
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|penems
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|carbapenems
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|cephems
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|carbacephems
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|oxacephems
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|monobactams
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|-
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|bgcolor="#FFFFFF"|[[File: T--Evry_Paris-Saclay--penam.png|center|100px]]   
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|bgcolor="#FFFFFF"|[[File: T--Evry_Paris-Saclay--carbapenam.png|center|100px]]
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|bgcolor="#FFFFFF"|[[File: T--Evry_Paris-Saclay--clavam.png|center|100px]]
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|bgcolor="#FFFFFF"|[[File: T--Evry_Paris-Saclay--penem.png|center|100px]] 
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|bgcolor="#FFFFFF"|[[File: T--Evry_Paris-Saclay--carbapenem.png|center|100px]]
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|bgcolor="#FFFFFF"|[[File: T--Evry_Paris-Saclay--cephem.png|center|100px]]
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|bgcolor="#FFFFFF"|[[File: T--Evry_Paris-Saclay--carbacephem.png|center|100px]] 
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|bgcolor="#FFFFFF"|[[File: T--Evry_Paris-Saclay--oxacephem.png|center|100px]] 
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|bgcolor="#FFFFFF"|[[File: T--Evry_Paris-Saclay--monobactam.png|center|60px]]
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|}
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Latest revision as of 20:19, 2 October 2023


AmpR

This part is AmpR, an E. coli gene coding for a β-lactamase enzyme. It is a derivative of the TEM-1 β-lactamase (Uniprot P62593) with two mutations V82I and V182A. In addition, compared to the sequence found in pSB1A3 from which it was derived by PCR amplification, two synonymous mutations Y44 (TAT->TAC) and S240 (TCC->TCG) were detected.

Usage and Biology

AmpR is a protein associated with the resistance mechanism against the large family of β-lactams, antibiotics which include the well known penicillin and ampicillin.

β-Lactamases act by hydrolysing the β-lactam ring (Figure 1), thus inactivating the antibiotic.

T--Evry Paris-Saclay--beta-lactam ring hydrolysis.png

Figure 1. The mode of action of β-lactamases: hydrolysis of the β-lactam ring.

Fortunately, among all the subgroups of β-lactams (table 1), some have less affinity with the β-lactamase(s) and overcome this resistance.

Table 1. β-lactam groups and sub-groups, classified based on their core ring structures.
penams carbapenams clavams penems carbapenems cephems carbacephems oxacephems monobactams
T--Evry Paris-Saclay--penam.png
T--Evry Paris-Saclay--carbapenam.png
T--Evry Paris-Saclay--clavam.png
T--Evry Paris-Saclay--penem.png
T--Evry Paris-Saclay--carbapenem.png
T--Evry Paris-Saclay--cephem.png
T--Evry Paris-Saclay--carbacephem.png
T--Evry Paris-Saclay--oxacephem.png
T--Evry Paris-Saclay--monobactam.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]