Difference between revisions of "Part:BBa K4586018"
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CD8 alpha is signal peptide translocate the conjugated protein to the cellular membrane. | CD8 alpha is signal peptide translocate the conjugated protein to the cellular membrane. | ||
| + | ==Literature Characterization== | ||
| + | The study created a reporter construct by joining the C-terminus of CD63, one of the most used exosome markers, to nanoluc (nluc), a tiny and potent bioluminescence reporter10. After progressive centrifugation to eliminate masking signals12, luminescence in the cell-culture supernatant was measured. This reporter gene was co-transfected with plasmids expressing potential candidates for exosome production augmentation. | ||
| + | <html><div align="center"style="border:solid #17252A; width:50%;float:center;"><img style=" max-width:850px; | ||
| + | width:75%; | ||
| + | height:auto; | ||
| + | position: relative; | ||
| + | top: 50%; | ||
| + | left: 35%; | ||
| + | transform: translate( -50%); | ||
| + | padding-bottom:25px; | ||
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| + | "src="https://static.igem.wiki/teams/4586/wiki/literature-characterisation-parts/cd8-alpha.png"> | ||
| + | <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span | ||
| + | Poly(A) RNA samples from each cell line were separated by gel electrophoresis, transferred to a nitrocellulose membrane, and hybridized with 32P-labeled cDNA probes specific for MC-CPA or CD8amp;. The membranes were then reprobed with a 32P-labeled p-actin cDNA probe to verify that equal amounts of RNA had been loaded in each lane. | ||
| + | The results showed that MC-CPA mRNA was expressed at a higher level in MW9.11-3 cells than in MC/9.IL-4 cells, while CDalpha mRNA was expressed at a similar level in both cell lines. These findings suggest that MC-CPA and CDalpha may play different roles in the development and differentiation of these two cell | ||
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| + | </span></p></div></html> | ||
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| + | ==References== | ||
| + | Hara, T., Harada, N., Mitsui, H., Miura, T., Ishizaka, T., & Miyajima, A. (1994). Characterization of cell phenotype by a novel cDNA library subtraction system: expression of CD8 alpha in a mast cell-derived interleukin-4-dependent cell line. | ||
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Revision as of 04:24, 25 September 2023
CD 8 alpha signal
Description
CD8 alpha is signal peptide translocate the conjugated protein to the cellular membrane.
Literature Characterization
The study created a reporter construct by joining the C-terminus of CD63, one of the most used exosome markers, to nanoluc (nluc), a tiny and potent bioluminescence reporter10. After progressive centrifugation to eliminate masking signals12, luminescence in the cell-culture supernatant was measured. This reporter gene was co-transfected with plasmids expressing potential candidates for exosome production augmentation.
References
Hara, T., Harada, N., Mitsui, H., Miura, T., Ishizaka, T., & Miyajima, A. (1994). Characterization of cell phenotype by a novel cDNA library subtraction system: expression of CD8 alpha in a mast cell-derived interleukin-4-dependent cell line.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]