Difference between revisions of "Part:BBa K187003:Design"

 
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<partinfo>BBa_K187003 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The 1% promoter produced ~1% of the expression level from a consensus promoter. The pAB plasmid adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes RFC for details on this method.  
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The 1% promoter produces ~1% of the expression level from a consensus promoter. The pAB plasmid adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes RFC for details on this method.
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===Source===
 
===Source===

Revision as of 02:28, 17 October 2009

Sigma 70 1% promoter in pAB BioBytes plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 47
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 1% promoter produces ~1% of the expression level from a consensus promoter. The pAB plasmid adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes RFC for details on this method.

Source

1% promoter was modified from Anderson collection of promoters, part BBa_J23113 pAB was derived from pUC19

References