Difference between revisions of "Part:BBa K4585000"

 
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A protein of truncated gonadotropin-releasing hormone.
 
A protein of truncated gonadotropin-releasing hormone.
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<h2 class="pageContent-main__title">
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                <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title-->
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                Insulin
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            <div class="pageContent-main__textBox">
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                <p>We have proved that Insulin can be processed and secreted outside the cell in 293T cells.
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                </p>
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                <!--put text here, <p>content</p>-->
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                1.Pattern diagram
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            </h2>
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            <div class="pageContent-main__textBox">
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                <p style="text-align: center;">
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/gnrh.png"></p>
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                <!--put image's url here-->
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of 9XUAS-Insulin</p>
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            </div>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                <!--class="pageContent-main__title" means it is the sub title-->
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                2.Experiment
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            </h2>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                <!--class="pageContent-main__title" means it is the sub title-->
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                2.1 Method
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            <div class="pageContent-main__textBox">
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                <!--all the content must included in this div-->
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                <p>In the experiment, we used an ELIAS kit specially designed to detect human mature insulin to detect cell eliquidation.
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                </p>
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                <!--put text here, <p>content</p>-->
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            </div>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                2.2 Results
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            </h2>
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            <div class="pageContent-main__textBox">
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                <!--all the content must included in this div-->
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                <p>Insulin can be processed and secreted outside the cell in 293T cells.
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                </p>
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                <p style="text-align: center;">
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                    <img width="400px" src="https://2021.igem.org/wiki/images/5/58/T--CSU_CHINA--tupian30.png"></p>
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                <!--put image's url here-->
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Standard curve of absorbance and insulin concentration</p>
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                <p style="text-align: center;">
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                    <img width="400px" src="https://2021.igem.org/wiki/images/5/57/T--CSU_CHINA--tupian31.png"></p>
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                <!--put image's url here-->
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.3 Insulin concentration in supernatant after blue light irradiation and dark treatment respectively</p>
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                <p style="text-align: center;">
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                    <img width="400px" src="https://2021.igem.org/wiki/images/6/6c/T--CSU_CHINA--tupian32.png"></p>
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                <!--put image's url here-->
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.4 Insulin concentration in supernatant (excluding insulin in medium) after blue light irradiation and dark treatment respectively</p>
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            </div>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                3.Caution
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            <div class="pageContent-main__textBox">
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                <p>Insulin is not suitable for long time preservation under the best culture temperature of cells (37℃). In the mean time, cells would also consume some of the expressed insulin. So the best tst time for Insulin is approximately 24 hours
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                    after transfection, it is relatively shorter than the test interval of LUC.
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                </p>
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            </div>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                Reference：
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                <p>[1]Mingqi Xie, Haifeng Ye, Hui Wang.β-cell-mimetic designer cells provide closed-loop glycemic control[J].Science.2016 Dec 9;354(6317):1296-1301.
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                </p>
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            </div>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:13, 24 September 2023


GnRH-C

A protein of truncated gonadotropin-releasing hormone.

Insulin

We have proved that Insulin can be processed and secreted outside the cell in 293T cells.

1.Pattern diagram

Fig.1 The model diagram of 9XUAS-Insulin

2.Experiment

2.1 Method

In the experiment, we used an ELIAS kit specially designed to detect human mature insulin to detect cell eliquidation.

2.2 Results

Insulin can be processed and secreted outside the cell in 293T cells.

Fig.2 Standard curve of absorbance and insulin concentration

Fig.3 Insulin concentration in supernatant after blue light irradiation and dark treatment respectively

Fig.4 Insulin concentration in supernatant (excluding insulin in medium) after blue light irradiation and dark treatment respectively

3.Caution

Insulin is not suitable for long time preservation under the best culture temperature of cells (37℃). In the mean time, cells would also consume some of the expressed insulin. So the best tst time for Insulin is approximately 24 hours after transfection, it is relatively shorter than the test interval of LUC.

Reference：

[1]Mingqi Xie, Haifeng Ye, Hui Wang.β-cell-mimetic designer cells provide closed-loop glycemic control[J].Science.2016 Dec 9;354(6317):1296-1301.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]