Difference between revisions of "Part:BBa K4656004"
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− | The Plee promotor is under the control of PchA | + | The Plee promotor is under the control of PchA. We designed and compared Ppcha-pcha-Plee-egfp and Ppcha-pcha-egfp circuit. |
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===Experimental results=== | ===Experimental results=== | ||
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Revision as of 13:04, 5 October 2023
PLEE1
PLEE1 is the promoter of the locus of enterocyte effacement which binds to pchA.
Since the specific binding site of pchA and PLEE1 is still unknown, at present, researchers have purchased Escherichia coli genome from atcc and amplified PLEE1 with different fragments by PCR as candidates, and constructed 6 different kinds of plasmids. +1 is the transcription start site TSS.
"Additionally, the best performing plasmid, pC4S-P4, contains a small part of the ler gene in the PLEE1 promoter region."[1] {The pC4S-P4: pDMB,PpchA-pchA-PLEE1(-98+218bp)-gfp;CmR}
Usage and Biology
The Plee promotor is under the control of PchA. We designed and compared Ppcha-pcha-Plee-egfp and Ppcha-pcha-egfp circuit.
Experimental results
![](https://static.igem.wiki/teams/4656/wiki/part-plee1.jpg)
![](https://static.igem.wiki/teams/4656/wiki/part-plee2.jpg)
![](https://static.igem.wiki/teams/4656/wiki/part-plee3.jpg)
![](https://static.igem.wiki/teams/4656/wiki/part-plee4.jpg)
Reference
[1]. Schwan W. R. (2011). Regulation of fim genes in uropathogenic Escherichia coli. World journal of clinical infectious diseases, 1(1), 17–25. https://doi.org/10.5495/wjcid.v1.i1.17.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]