Difference between revisions of "Part:BBa K4656004"
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E. coli strains transfected with either A segment or B segment were cultivated in the presence of 10mM and 20mM sodium butyrate. The results revealed that the sensitivity of A segment to sodium butyrate and its ability to enhance the expression of downstream genes were superior to those of B segment. | E. coli strains transfected with either A segment or B segment were cultivated in the presence of 10mM and 20mM sodium butyrate. The results revealed that the sensitivity of A segment to sodium butyrate and its ability to enhance the expression of downstream genes were superior to those of B segment. | ||
− | In the end, we opted for the design of the A | + | In the end, we opted for the design of the A segment,which involves plee promotor. |
===Reference=== | ===Reference=== |
Revision as of 13:21, 22 September 2023
PLEE1
PLEE1 is the promoter of the locus of enterocyte effacement which binds to pchA.
Since the specific binding site of pchA and PLEE1 is still unknown, at present, researchers have purchased Escherichia coli genome from atcc and amplified PLEE1 with different fragments by PCR as candidates, and constructed 6 different kinds of plasmids. +1 is the transcription start site TSS.
"Additionally, the best performing plasmid, pC4S-P4, contains a small part of the ler gene in the PLEE1 promoter region."[1] {The pC4S-P4: pDMB,PpchA-pchA-PLEE1(-98+218bp)-gfp;CmR}
Usage and Biology
The Plee promotor is under the control of PchA, we designed and compared Ppcha-pcha-Plee-egfp and Ppcha-pcha-egfp circuit.
Experiment results
![](https://static.igem.wiki/teams/4656/wiki/part-plee1.jpg)
![](https://static.igem.wiki/teams/4656/wiki/part-plee2.jpg)
![](https://static.igem.wiki/teams/4656/wiki/part-plee3.jpg)
![](https://static.igem.wiki/teams/4656/wiki/part-plee4.jpg)
Reference
[1]. Schwan W. R. (2011). Regulation of fim genes in uropathogenic Escherichia coli. World journal of clinical infectious diseases, 1(1), 17–25. https://doi.org/10.5495/wjcid.v1.i1.17.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]