Difference between revisions of "Part:BBa K283003"
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<partinfo>BBa_K283003 short</partinfo>
 
<partinfo>BBa_K283003 short</partinfo>
  
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This part generates the chez protein which is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. A 6-histidine tail is added to the chez coding region. The pTet promoter can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor.  
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This part was designed to generate the cheZ proteins which are responsible for the dephosphorylation of cheY protein involved in bacteria flagella movement. A 6-histidine tail is added to the cheZ encoding region. The pTet can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor. The backbone of this biobrick is based on BBa_J61003.  CheZ_his tag and strong double terminators lie between XbaI and SpeI.
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<!-- Add more about the biology of this part here
 
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Revision as of 12:58, 20 October 2009

pTet-rbs-chez_histag-double terminator

This part was designed to generate the cheZ proteins which are responsible for the dephosphorylation of cheY protein involved in bacteria flagella movement. A 6-histidine tail is added to the cheZ encoding region. The pTet can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor. The backbone of this biobrick is based on BBa_J61003. CheZ_his tag and strong double terminators lie between XbaI and SpeI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 726
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]