Difference between revisions of "Part:BBa K4586019"

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==Usage==
 
==Usage==
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We used this basic part in our design to mediate the transfer of our transcription module VP64 into the nucleus to carry  out its action on the target promoter that regulates the expression of our therapeutic agent (Cass12k).
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This part implemented in our design is a linker peptide to link between the elements of our internal domain the syn notch receptor which are the zinc finger peptide (ZF21.16) and, SV40 NLS and VP64 that represent our potent transcription module.
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Revision as of 10:11, 21 September 2023


G4S linker

Part Description

This part codes for the poly-Glycine-Serine (G4S) linker peptide that is a type of flexible,unstructured synthetic peptide linker sequence. G4S linker is composed of a core pentapeptide Gly-Gly-Gly-Gly-Ser that is repeated and usually found within scFV-based synthetic receptors as CARs and syn notch receptors.

Usage

This part implemented in our design is a linker peptide to link between the elements of our internal domain the syn notch receptor which are the zinc finger peptide (ZF21.16) and, SV40 NLS and VP64 that represent our potent transcription module.





Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]