Difference between revisions of "Part:BBa K4890015:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Our team aims to detect the amounts of heavy metals by transgenic fruit flies. We plan to insert MTF-1 gene driven by UAS and GFP gene driven by MRE in Drosophila embryos, S2 cells, respectively. These two transgenic Drosophila lines interbreed and the offspring with both MTF-1 and GFP genes cross with the Drosophila with genotype GMR-GAL4. In this way, we constructed a Drosophila line with genotype UAS-MTF-1;MRE-GFP/GMR-GAL4. The line can be used as a visual monitoring system for heavy metals. In response to different heavy metal concentrations in the growing environment, the Drosophila shows green fluorescence in the eyes.
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===Source===
 
===Source===
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MRE sequence is based on BBa_K4890002.
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Hsp70 sequence is based on BBa_K4890004.
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GFP gene is based on BBa_S03452.
  
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===References===
 
===References===

Latest revision as of 10:42, 5 October 2023


MRE-Hsp70-GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 196
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our team aims to detect the amounts of heavy metals by transgenic fruit flies. We plan to insert MTF-1 gene driven by UAS and GFP gene driven by MRE in Drosophila embryos, S2 cells, respectively. These two transgenic Drosophila lines interbreed and the offspring with both MTF-1 and GFP genes cross with the Drosophila with genotype GMR-GAL4. In this way, we constructed a Drosophila line with genotype UAS-MTF-1;MRE-GFP/GMR-GAL4. The line can be used as a visual monitoring system for heavy metals. In response to different heavy metal concentrations in the growing environment, the Drosophila shows green fluorescence in the eyes.


Source

MRE sequence is based on BBa_K4890002. Hsp70 sequence is based on BBa_K4890004. GFP gene is based on BBa_S03452.


References